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RNA Library Construction and Deep Sequencing of Apple and Peach

General Information

Document Type:PRESOL
Posted Date:Aug 23, 2017
Category: Special Studies and Analyses - Not R&D
Set Aside:N/A

Contracting Office Address

Agricultural Research Service - Northeast Area

Description

RNA Library Construction and Deep Sequencing of Apple and Peach This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotations are being requested and a written solicitation will not be issued. Solicitation number AG349BS177628 is being issued as a request for quotation. The solicitation documents and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-95. This requirement is a total small business set-aside under NAICS 541711, Size standard is 1000 employees. Contractors are responsible for reading and complying with this entire combined synopsis/solicitation and monitoring website, http://www.fbo.gov for any additional information relating to this requirement. Submission of quotations to include all information requested shall be in accordance with the instructions identified in this document. Small Businesses with the demonstrated capability and financial capacity to provide specified services in accordance with the specified delivery schedule are invited to respond to this request. Contractor shall provide all services, supplies, equipment and facilities necessary to provide the line items specified in accordance with the terms, conditions, and specifications contained in this document. Statement of Work: The USDA ARS AFRS requires RNA Library Construction, Quality Control and Deep Sequencing of 117 RNAs utilizing paired end 150 bases to be pooled and sequenced yielding >30 million reads per library by Illumina HiSeq or equivalent in accordance with the following: Performance Elements and Benchmarks: 1. Verification of RNA quality for RNAs submitted. 2. Construction of RNA library using polyA selected RNAs from different plant tissues and verification of library quality for each of the libraries. Substandard library quality will require library(s) to be reconstructed. 3. Libraries will be pooled for sequencing in such a way to avoid batch effects and each library within the pool must be given a unique barcode. Data provided must be separated by barcodes for pooled lanes. 4. Libraries will be subjected to a minimum of paired-end 150 base sequencing to yield a minimum of 30 million paired end reads per library for the following: Line Item 0001 - Library construction (PolyA selected) from apple hypanthium, ovary wall and seed total RNA, 3 biological replicates, 3 tissues, 3 treatments - 27 libraries. (RNAs provided within one week). Line Item 0002 - Library construction (PolyA selected) from total RNA of peach ovule/seed, hypanthium, pedicel and ovary wall tissues - 21 libraries. (RNAs provided within one week of award). Line Item 0003 - Library construction (PolyA selected) from total RNA of apple ovule/seed, hypanthium, pedicel and ovary wall tissues - 21 libraries. (RNAs provided within one week of award). Line Item 0004 - Library construction (PolyA selected) from total RNA of peach embryo, nucellus, seed coat, seed, and embryo sac tissues - 24 libraries. (RNAs provided within two weeks of award). Line Item 0005 - Library construction (PolyA selected) from total RNA of apple embryo, nucellus, seed coat, seed, and embryo sac tissues - 24 libraries. (RNAs provided within two weeks of award). 5. Data quality must be the Illumina specification or equal that 75% of the nucleotides are greater than Q30 for 150bp sequencing. 6. Deliverables: For Each Line Item: Data will be stored as bar-code de-convoluted and labeled.fastq files available on a secure site or on a hard drive and shall be provided FOB Destination to USDA-ARS-AFRS 2217 Wiltshire Road, Kearneysville, WV 25430 within 60 days of receipt of Government Samples. Schedule Line Items: Line Item 01 - 27 total RNA samples from apple$_________ Line Item 02 - 21 total RNA samples from peach$_________ Line Item 03 - 21 total RNA samples from apple$_________ Line Item 04 - 24 total RNA samples from peach$_________ Line Item 05 - 24 total RNA samples from apple$_________ INSTRUCTIONS FOR SUBMISSION OF QUOTATION - Quotations shall be submitted to the attention of: Timothy Smearman, USDA, AFRS, Administration Office, 2217 Wiltshire Road, Kearneysville, WV 25430 / tim.smearman@ars.usda.gov and be received prior to 4:00 P.M. (EST) on September 5, 2017. ** OFFERORS SHALL SUBMIT THE FOLLOWING: (a) Submit documentation to show technical capability to meet or exceed Performance Elements and Benchmarks. (b) Submit Firm Fixed Price Quotation for each of the Schedule Line Items and Total Price. (c) Submit two separate Past Performance References with contact information for two recent contracts (within past year) of contracts similar to the scope of this requirement using Illumina HiSeq or equivalent. To be eligible for an award, contractor must be a Small Business under NAICS Code 541711 and have current and complete registration in the System for Award Management www.sam.gov. Offeror certifies, by submission of quotation, that registration in SAM is current to include Online Representations and Certifications as of submission date of quotation. Pending availability of funding, the basis for any potential award is Lowest Price Technically Acceptable (LPTA). "LPTA" means the expected outcome of the acquisition that, results from selection of the lowest evaluated price (evaluated as total cost of all line items) technically acceptable quotation that fully meets all specifications and having satisfactory past performance. Only the lowest priced quote will be evaluated for technical acceptability. The next lowest offer will only be evaluated if the aforementioned quote is not found technically acceptable (and so on). Only one award will be made under this solicitation based on availability of funding. The following FAR and AGAR provisions and clauses apply to this acquisition and are available in full text at: http://farsite.hill.af.mil/ 52.204-7 System for Award Management; 52.204-10 Reporting Executive Compensation and First-Tier Subcontract Awards; 52.209-6 Protecting the Governments Interest When Subcontracting with Contractors Debarred, Suspended, or Proposed for Debarment; 52.209-10 Prohibition on Contracting with Inverted Domestic Corporations; 52.211-6 Brand Name or Equal; 52.212-1 Instructions to Offers-Commercial Items; 52.212-2 Evaluation-Commercial Items; 52.212-4 Contract Terms and Conditions - Commercial Items; 52.212-5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders; 52.219-6 Notice of Total Small Business Set-Aside; 52.219-13 Notice of Set-Aside of Orders; 52.219-28 Post Award Small Business Program Representation; 52.222-3 Convict Labor; 52.222-19 Child Labor Cooperation with Authorities and Remedies; 52.222-21 Prohibition of Segregated Facilities; 52.222-26 Equal Opportunity; 52.222-36 Equal Opportunity for Workers with Disabilities; 52.222-50 Combating Trafficking in Persons; 52.223-18 Encouraging Contractor Policies to Ban Text Messaging while Driving; 52.225-3 Buy American--Free Trade Agreements--Israeli Trade Act; 52.225-4 Buy American -- Free Trade Agreements - Israeli Trade Act Certificate; 52.225-13 Restrictions on Certain Foreign Purchases; 52.225-25 Prohibitions on Contracting with Entities Engaging in Certain Activities or Transactions Relating to Iran; 52.232-18 Availability of Funds; 52.232-24 Prohibition of Assignment of Claims; 52.232-25 Prompt Payment; 52.232-39 Unenforceability of Unauthorized Obligations; 52.232-33 Payment by Electronic Funds Transfer-System for Award Management; 52.233-1 Disputes; 52.233-3 Protest after Award; 52.233-4 Applicable Law for Breach of Contract Claim; 52.242-13 Bankruptcy; 52.242-17 Government Delay of Work; 52.246-1 Contractor Inspection Requirements; 52.247-34 F.o.b. Destination; 52.249-8 Default (Fixed Price Supply and Service); 52.249-1 Termination for Convenience of the Government (Fixed-Price) (Short Form); 52.252-1 Solicitations Provisions Incorporated by Reference; 52.252-2 Clauses Incorporated by Reference; 452.204-70 Inquiries; 452.219-70 Size Standard and NAICS Code Information.

Original Point of Contact

POC TIMOTHY A SMEARMAN, Phone: 304-725-3451 EXT 341

Place of Performance

Address:
Kearneysville, West Virginia, 25430, United States
25430,
Link: FBO.gov Permalink
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